| Registro completo |
| Provedor de dados: |
ArchiMer
|
| País: |
France
|
| Título: |
Calcium response of KCl-excited populations of ventricular myocytes from the European sea bass (Dicentrarchus labrax): a promising approach to integrate cell-to-cell heterogeneity in studying the cellular basis of fish cardiac performance
|
| Autores: |
Ollivier, Helene
Marchant, James
Le Bayon, Nicolas
Servili, Arianna
Claireaux, Guy
|
| Data: |
2015-10
|
| Ano: |
2015
|
| Palavras-chave: |
Sea bass
Myocyte populations
Calcium signaling
Cardiac plasticity
Inter-individual variability
|
| Resumo: |
Climate change challenges the capacity of fishes to thrive in their habitat. However, through phenotypic diversity, they demonstrate remarkable resilience to deteriorating conditions. In fish populations, inter-individual variation in a number of fitness-determining physiological traits, including cardiac performance, is classically observed. Information about the cellular bases of inter-individual variability in cardiac performance is scarce including the possible contribution of excitation-contraction (EC) coupling. This study aimed at providing insight into EC coupling-related Ca2+ response and thermal plasticity in the European sea bass (Dicentrarchus labrax). A cell population approach was used to lay the methodological basis for identifying the cellular determinants of cardiac performance. Fish were acclimated at 12 and 22 A degrees C and changes in intracellular calcium concentration ([Ca2+](i)) following KCl stimulation were measured using Fura-2, at 12 or 22 A degrees C-test. The increase in [Ca2+](i) resulted primarily from extracellular Ca2+ entry but sarcoplasmic reticulum stores were also shown to be involved. As previously reported in sea bass, a modest effect of adrenaline was observed. Moreover, although the response appeared relatively insensitive to an acute temperature change, a difference in Ca2+ response was observed between 12- and 22 A degrees C-acclimated fish. In particular, a greater increase in [Ca2+](i) at a high level of adrenaline was observed in 22 A degrees C-acclimated fish that may be related to an improved efficiency of adrenaline under these conditions. In conclusion, this method allows a rapid screening of cellular characteristics. It represents a promising tool to identify the cellular determinants of inter-individual variability in fishes' capacity for environmental adaptation.
|
| Tipo: |
Text
|
| Idioma: |
Inglês
|
| Identificador: |
https://archimer.ifremer.fr/doc/00283/39412/37904.pdf
DOI:10.1007/s00360-015-0924-6
https://archimer.ifremer.fr/doc/00283/39412/
|
| Editor: |
Springer Heidelberg
|
| Formato: |
application/pdf
|
| Fonte: |
Journal Of Comparative Physiology B-Biochemical Systemic And Environmental Physiology (0174-1578) (Springer Heidelberg), 2015-10 , Vol. 185 , N. 7 , P. 755-765
|
| Direitos: |
Springer-Verlag Berlin Heidelberg 2015
info:eu-repo/semantics/openAccess
restricted use
|
|
